pMKO.1 GFP B56 gamma3 shRNA 14-1 | Addgene 10681 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMKO.1 GFP B56 gamma3 shRNA 14-1

catalog :

10681

citations: 3

Reference
Watt L, Panicker N, Mannan A, Copeland B, Kahl R, Dun M, et al. Functional importance of PP2A regulatory subunit loss in breast cancer. Breast Cancer Res Treat. 2017;166:117-131 pubmed publisher
Azad A, Fox J, Leverrier S, Storey A. Blockade of the BAK hydrophobic groove by inhibitory phosphorylation regulates commitment to apoptosis. PLoS ONE. 2012;7:e49601 pubmed publisher
Chen W, Possemato R, Campbell K, Plattner C, Pallas D, Hahn W. Identification of specific PP2A complexes involved in human cell transformation. Cancer Cell. 2004;5:127-36 pubmed

product information

Catalog Number :

10681

Product Name :

pMKO.1 GFP B56 gamma3 shRNA 14-1

article :

doi	10.1016/S1535-6108(04)00026-1
id	364
pubmed_id	14998489

bacterial resistance :

Ampicillin

cloning :

backbone

pMKO.1

backbone_mutation

backbone_origin

backbone_size

6700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

RNAi

growth notes :

To make retroviruses expressing shRNAs, oligonucleotides were inserted in the vector pMKO.1-puro modified by replacing the puromycin resistance gene with hrGFP (pMKO.1-GFP). The shRNAs were generated by cloning nucleotides 423-442 of PP2A B56 gamma followed by a 6 bp loop and the corresponding antisense sequence followed by five thymidines into pMKO.1-GFP.

origin :

pi :

alt_names

PP2A B56 gamma

B56 gamma

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	pLXSN 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	B56G, B56gamma, PR61G
gene	PPP2R5C
id	5527

genbank_ids

mutation

name

PP2A B56 gamma3 shRNA

shRNA_sequence

size

species

9606

Homo sapiens