pMKO.1 GFP B55 alpha shRNA 3-1 | Addgene 10679 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMKO.1 GFP B55 alpha shRNA 3-1

catalog :

10679

citations: 3

Reference
Watt L, Panicker N, Mannan A, Copeland B, Kahl R, Dun M, et al. Functional importance of PP2A regulatory subunit loss in breast cancer. Breast Cancer Res Treat. 2017;166:117-131 pubmed publisher
Lin S, Ptasinska A, Chen X, Shrestha M, Assi S, Chin P, et al. A FOXO1-induced oncogenic network defines the AML1-ETO preleukemic program. Blood. 2017;130:1213-1222 pubmed publisher
Chen W, Possemato R, Campbell K, Plattner C, Pallas D, Hahn W. Identification of specific PP2A complexes involved in human cell transformation. Cancer Cell. 2004;5:127-36 pubmed

product information

Catalog Number :

10679

Product Name :

pMKO.1 GFP B55 alpha shRNA 3-1

article :

doi	10.1016/S1535-6108(04)00026-1
id	364
pubmed_id	14998489

bacterial resistance :

Ampicillin

cloning :

backbone

pMKO.1

backbone_mutation

backbone_origin

backbone_size

6700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

RNAi

growth notes :

To make retroviruses expressing shRNAs, oligonucleotides were inserted in the vector pMKO.1-puro modified by replacing the puromycin resistance gene with hrGFP (pMKO.1-GFP). The shRNAs were generated by cloning nucleotides 1156-1175 of PP2A B55 alpha followed by a 6 bp loop and the corresponding antisense sequence followed by five thymidines into pMKO.1-GFP.

origin :

pi :

alt_names

PP2A B55 alpha

B55 alpha

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	pLXSN 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	B55A, B55ALPHA, PR52A, PR55A, PR55alpha
gene	PPP2R2A
id	5520

genbank_ids

mutation

name

PP2A B55 alpha shRNA

shRNA_sequence

size

species

9606

Homo sapiens