This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe HcRed Ras
catalog :
10678
citations: 2
Reference
Roof A, Trudeau T, Gutierrez Hartmann A. Pituitary somatolactotropes evade an oncogenic response to Ras. Mol Cell Endocrinol. 2018;476:165-172 pubmed publisher
Boehm J, Hession M, Bulmer S, Hahn W. Transformation of human and murine fibroblasts without viral oncoproteins. Mol Cell Biol. 2005;25:6464-74 pubmed
product information
Catalog Number :
10678
Product Name :
pBabe HcRed Ras
article :
doi10.1128/MCB.25.15.6464-6474.2005
id361
pubmed_id16024784
bacterial resistance :
Ampicillin
cloning :
backbonepBabe HcRed
backbone_mutation
backbone_origin
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
pBabe-puro-Ras was digested with ClaI, blunted and purified. Linearized vector was digested with HindIII, releasing the 5.6kb vector piece. pcDNA-HcRed was digested with HindIII and PmeI, and the 700bp piece containing HcRed was ligated into pBabe.
origin :
37
pi :
alt_names
v-Ha-ras
Hras
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaBI
cloning_site_5SnaBI
promoter
sequencing_primer_3
sequencing_primer_5pBabe5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutationG12V, constitutively active
nameHras V12
shRNA_sequence
size1200
species
9606
Homo sapiens
tags
resistance markers :
184
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA