This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMKO.1 puro Rb shRNA
catalog :
10670
citations: 5
Reference
Geisinger J, Stearns T. CRISPR/Cas9 treatment causes extended TP53-dependent cell cycle arrest in human cells. Nucleic Acids Res. 2020;48:9067-9081 pubmed publisher
Yang Z, Maciejowski J, de Lange T. Nuclear Envelope Rupture Is Enhanced by Loss of p53 or Rb. Mol Cancer Res. 2017;15:1579-1586 pubmed publisher
Salvati E, Rizzo A, Iachettini S, Zizza P, Cingolani C, D Angelo C, et al. A basal level of DNA damage and telomere deprotection increases the sensitivity of cancer cells to G-quadruplex interactive compounds. Nucleic Acids Res. 2015;43:1759-69 pubmed publisher
Kumar P P, Emechebe U, Smith R, Franklin S, Moore B, Yandell M, et al. Coordinated control of senescence by lncRNA and a novel T-box3 co-repressor complex. elife. 2014;3: pubmed publisher
Boehm J, Hession M, Bulmer S, Hahn W. Transformation of human and murine fibroblasts without viral oncoproteins. Mol Cell Biol. 2005;25:6464-74 pubmed
product information
Catalog Number :
10670
Product Name :
pMKO.1 puro Rb shRNA
article :
doi10.1128/MCB.25.15.6464-6474.2005
id361
pubmed_id16024784
bacterial resistance :
Ampicillin
cloning :
backbonepMKO.1
backbone_mutation
backbone_origin
backbone_size6700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
and reverse, 5'-AATTCAAAAACAGAGATCGTGTATTGAGATTCTCGAGAATCTCAATACACGATCTCTG-3'
5'-CCGGCAGAGATCGTGTATTGAGATTCTCGAGAATCT
CAATACACGATCTCTGTTTTTG-3';
Sequences specific for 3'UTR of Rb transcript. forward,
origin :
37
pi :
alt_names
Rb
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pLXSN 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
geneRB1
id5925
genbank_ids
mutation
nameRb shRNA
shRNA_sequence
size58
species
9606
Homo sapiens
tags
resistance markers :
184
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA