PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B | Addgene 106309 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

catalog :

106309

citations: 1

Reference
Liu H, Dong P, Ioannou M, Li L, Shea J, Pasolli H, et al. Visualizing long-term single-molecule dynamics in vivo by stochastic protein labeling. Proc Natl Acad Sci U S A. 2018;115:343-348 pubmed publisher

product information

Catalog Number :

106309

Product Name :

PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

article :

doi	10.1073/pnas.1713895115
id	28192612
pubmed_id	29284749

bacterial resistance :

Ampicillin

cloning :

backbone

pBR322

backbone_mutation

backbone_origin

synthetic

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	MluI
promoter	EF1
sequencing_primer_3	5'-AAGACGGCAATATGGTGGAAAA-3'
sequencing_primer_5	5'-CCTACCTAGACTCAGC-3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	H2B.1, H2B/f, H2BFF, HIST1H2BB
gene	H2BC3
id	3018

genbank_ids

AF531285.1

mutation

name

6xflag-RT3-3xHaloTag(MtoL)-H2B

shRNA_sequence

size

3276

species

9606

Homo sapiens