This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AAV-U6-dgRNA-CAG-MPH
catalog :
106259
citations: 1
Reference
Liao H, Hatanaka F, Araoka T, Reddy P, Wu M, Sui Y, et al. In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. Cell. 2017;171:1495-1507.e15 pubmed publisher
product information
Catalog Number :
106259
Product Name :
AAV-U6-dgRNA-CAG-MPH
article :
doi10.1016/j.cell.2017.10.025
id28192593
pubmed_id29224783
bacterial resistance :
Ampicillin
cloning :
backboneAAV
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
CRISPR
growth notes :
MPH was constructed from plasmid lenti_MS2-P65-HSF1_Hygro (Addgene 61426)
growth strain :
Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone
origin :
30
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSTF1
geneHSF1
id3297
genbank_ids
mutation
nameMS2-P65-HSF1
shRNA_sequenceSapI
size
species
9606
Homo sapiens
100
Synthetic
tags
plasmid copy :
Addgene 61426
resistance markers :
24
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA