This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Opto-GPR42
catalog :
106022
citations: 1
Reference
Morri M, Sanchez Romero I, Tichy A, Kainrath S, Gerrard E, Hirschfeld P, et al. Optical functionalization of human Class A orphan G-protein-coupled receptors. Nat Commun. 2018;9:1950 pubmed publisher
product information
Catalog Number :
106022
Product Name :
Opto-GPR42
article :
doi10.1038/s41467-018-04342-1
id28192564
pubmed_id29769519
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1-
backbone_mutation
backbone_originInvitrogen
backbone_size5427
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
two step cloning; 5' and 3' sites cannot be used for (sub)cloning
growth strain :
Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR42; Opto-GPR42; B12)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterCMV
sequencing_primer_3pcDNA_R (CAACAGATGGCTGGCAAC)
sequencing_primer_5CMV_F (CGCAAATGGGCGGTAGGCGTG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFFAR1L, FFAR3L, GPR41L, GPR42P
geneGPR42
id2866
genbank_ids
mutationChimeric receptor protein
nameOpto-GPR42
shRNA_sequence
size1233
species
9606
Homo sapiens
9913
Bos taurus
tags
locationN terminal on insert
tagVSV-G
locationC terminal on insert
tagRhodopsin-1D4
resistance markers :
2022
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA