This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV iGluf
catalog :
105833
citations: 1
Reference
Helassa N, Dürst C, Coates C, Kerruth S, Arif U, Schulze C, et al. Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018;115:5594-5599 pubmed publisher
product information
Catalog Number :
105833
Product Name :
pCMV iGluf
article :
doi10.1073/pnas.1720648115
id28192540
pubmed_id29735711
bacterial resistance :
Ampicillin
cloning :
backbonepCMV(MinDis) [variant of pDisplay]
backbone_mutationFrom pDisplay: Hemagglutinin (HA) tag removed
backbone_originmodified from Invitrogen
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
pCMV iGluf is the E25D variant of iGluSnFR. (iGluSnFR was created by Marvin et al.2013) bioRxiv: https://doi.org/10.1101/233494
growth strain :
Fast indicator for extracellular glutamate imaging
origin :
37
pi :
alt_names
iGluf
cloning
clone_methodRestriction Enzyme
cloning_site_3PstI
cloning_site_5BglII
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
21239023
21239023
mutationChanged Glu 25 to Asp
nameiGluSnFR E25D variant
shRNA_sequence
size1569
species
32644
Other
Escherichia coli (strain K12)
tags
locationC terminal on insert
tagPDGFR
locationC terminal on insert
tagMyc
plasmid copy :
pCMV(MinDis).iGluSnFR vector was obtained from Addgene
resistance markers :
2771
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA