This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
catalog :
105648
product information
Catalog Number :
105648
Product Name :
pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
article :
doi
id28192494
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepDESTTol2pA2
backbone_mutation
backbone_originChi-Bin Chien lab
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Addgene NGS results found additional amino acids GGGRSGNSRPLEPLDSAAL at the C-terminus of the EGFP translation. Plasmid was validated and confirmed to function as intended by the depositing laboratory
growth strain :
Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks
origin :
37
pi :
alt_names
isl3
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesISL-3, isl3, islet 3, zfIsl-3, zgc:109840
geneisl2b
id30151
genbank_ids
mutation
nameTol2-Isl2b:GFP:polyA
shRNA_sequence
size2000
species
7955
Danio rerio
32644
Other
tags
plasmid copy :
Derived from two plasmids from Chi-Bin Chien s lab at the University of Utah.
resistance markers :
3732
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA