This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEMHE-H2B-mEGFP
catalog :
105528
citations: 1
Reference
Clift D, McEwan W, Labzin L, Konieczny V, Mogessie B, James L, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017;171:1692-1706.e18 pubmed publisher
product information
Catalog Number :
105528
Product Name :
pGEMHE-H2B-mEGFP
article :
doi10.1016/j.cell.2017.10.033
id28192365
pubmed_id29153837
bacterial resistance :
Ampicillin
cloning :
backbonepGEMHE
backbone_mutation
backbone_origin
backbone_size3834
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
in vitro Transcription
growth strain :
T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA
origin :
37
pi :
alt_names
HIST1H2BJ
H2B/r, H2BFR, H2BJ
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SalI
promoterT7
sequencing_primer_3CGTCGCCGTCCAGCTCGACCAG
sequencing_primer_5AAGGCGATTAAGTTGGGTAACG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B/r, H2BFR, H2BJ, HIST1H2BJ
geneH2BC11
id8970
genbank_ids
NM_021058.3
mutation
nameH2B
shRNA_sequence
size390
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagmEGFP
resistance markers :
3776
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA