This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmKikGR Actin
catalog :
105315
citations: 1
Reference
Doyle A, Kutys M, Conti M, Matsumoto K, Adelstein R, Yamada K. Micro-environmental control of cell migration--myosin IIA is required for efficient migration in fibrillar environments through control of cell adhesion dynamics. J Cell Sci. 2012;125:2244-56 pubmed publisher
product information
Catalog Number :
105315
Product Name :
pmKikGR Actin
article :
doi10.1242/jcs.098806
id28192454
pubmed_id22328520
bacterial resistance :
Kanamycin
cloning :
backbonepmKikGRNB
backbone_mutation
backbone_originmodified pEGFP-C1
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
pmKikGR was constructed by inserting sequence corresponding to mKikGR with Kozak sequence to replace EGFP in Nhe1 /BglII sites of pEGFP NBC1. mKikGR was from Dr. Atsushi Miyawaki (Riken BSI).
growth strain :
Photoconvertable cytoskeletal protein actin to study kinetics or dynamics
origin :
37
pi :
alt_names
ACTB
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba1
cloning_site_5BamH1
promoter
sequencing_primer_3ATGTTTCAGGTTCAGGGGGAGGTG
sequencing_primer_5TCCAGTTGCCAGACTATCAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
mutation
nameactin beta
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagKikGR
resistance markers :
1793
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA