This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP Tubulin K40A
catalog :
105304
citations: 1
Reference
Joo E, Yamada K. MYPT1 regulates contractility and microtubule acetylation to modulate integrin adhesions and matrix assembly. Nat Commun. 2014;5:3510 pubmed publisher
product information
Catalog Number :
105304
Product Name :
pEGFP Tubulin K40A
article :
doi10.1038/ncomms4510
id19822
pubmed_id24667306
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Original pEGFP-Tub from Clontech. Mutation in Tubulin was introduced by using site-directed mutagenesis kit (Stratagene)
growth strain :
To study effects of acetylation-null tubulin
origin :
37
pi :
alt_names
TUBA1B
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesK-ALPHA-1
geneTUBA1B
id10376
genbank_ids
mutationK40A
namealpha tubulin
shRNA_sequence
size1350
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
1793
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA