This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRFP-NLS
catalog :
105296
citations: 1
Reference
Petrie R, Koo H, Yamada K. Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix. Science. 2014;345:1062-5 pubmed publisher
product information
Catalog Number :
105296
Product Name :
pRFP-NLS
article :
doi10.1126/science.1256965
id28192452
pubmed_id25170155
bacterial resistance :
Kanamycin
cloning :
backbonepERFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Human Rac1 sequence spanning nucleotides aa 171-192. The insert was from Dr. Natalie Lamarche-Vane (McGill University).
growth strain :
To visualize nuclear dynamics in living cells
origin :
37
pi :
alt_names
RAC1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5EcoR1
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
mutationaa encoding 172-192
nameRac1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagRFP
resistance markers :
1793
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA