pECFP-Cdc42-Y40C | Addgene 105295 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pECFP-Cdc42-Y40C

catalog :

105295

citations: 1

Reference
Petrie R, Gavara N, Chadwick R, Yamada K. Nonpolarized signaling reveals two distinct modes of 3D cell migration. J Cell Biol. 2012;197:439-55 pubmed publisher

product information

Catalog Number :

105295

Product Name :

pECFP-Cdc42-Y40C

article :

doi	10.1083/jcb.201201124
id	11625
pubmed_id	22547408

bacterial resistance :

Kanamycin

cloning :

backbone

pECFP-C1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Human Cdc42 sequence cloned with a nucleotide (C) added to the 5' end to keep it in frame. Amino acid Y at position 40 of the protein sequence is mutated to C. The insert was from Dr. Natalie Lamarche-Vane (McGill University).

growth strain :

Control for FRET biosensor analysis - cannot bind downstream effectors

origin :

pi :

alt_names

CDC42

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamH1
cloning_site_5	EcoR1
promoter
sequencing_primer_3
sequencing_primer_5	CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDC42Hs, G25K, TKS
gene	CDC42
id	998

genbank_ids

mutation

Y40C

name

Cdc42

shRNA_sequence

size

species

9606

Homo sapiens