This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pECFP-Rac1Q61L
catalog :
105292
citations: 2
Reference
Powers R, Daza R, Koehler A, Courchet J, Calabrese B, Hevner R, et al. Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1. Mol Biol Cell. 2022;33:ar64 pubmed publisher
Petrie R, Gavara N, Chadwick R, Yamada K. Nonpolarized signaling reveals two distinct modes of 3D cell migration. J Cell Biol. 2012;197:439-55 pubmed publisher
product information
Catalog Number :
105292
Product Name :
pECFP-Rac1Q61L
article :
doi10.1083/jcb.201201124
id11625
pubmed_id22547408
bacterial resistance :
Kanamycin
cloning :
backbonepECFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Human Rac1 sequence cloned with a nucleotide (C) added to the 5' end to keep it in frame. Amino acid Q at position 61 of the protein sequence is mutated to L. The insert was from Dr. Natalie Lamarche-Vane (McGill University).
growth strain :
Constitutively active Rac 1
origin :
37
pi :
alt_names
RAC1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5EcoR1
promoter
sequencing_primer_3
sequencing_primer_5CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
mutationQ61L
nameRac1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagCFP
resistance markers :
1793
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA