HR_G3BP1-V5-APEX2-GFP | Addgene 105284 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

HR_G3BP1-V5-APEX2-GFP

catalog :

105284

citations: 3

Reference
Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, D xfc rnberger G, et al. Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. elife. 2023;12: pubmed publisher
Elmsaouri S, Markmiller S, Yeo G. APEX Proximity Labeling of Stress Granule Proteins. Methods Mol Biol. 2022;2428:381-399 pubmed publisher
Markmiller S, Soltanieh S, Server K, Mak R, Jin W, Fang M, et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell. 2018;172:590-604.e13 pubmed publisher

product information

Catalog Number :

105284

Product Name :

HR_G3BP1-V5-APEX2-GFP

article :

doi	10.1016/j.cell.2017.12.032
id	28192451
pubmed_id	29373831

bacterial resistance :

Ampicillin

cloning :

backbone

HR120PA-1

backbone_mutation

inserted PGK-DTA-pA negative selection cassette downstream of 3' arm of homology.

backbone_origin

Systems Biosciences

backbone_size

2668

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

human homologous recombination donor

growth strain :

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13R
sequencing_primer_5	M13 (-40) Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	G3BP, HDH-VIII
gene	G3BP1
id	10146

genbank_ids

mutation

name

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette

shRNA_sequence

size

8004

species

9606

Homo sapiens

32644

Other

soybean