This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YIplac211-SEC7-mCherry2Bx6
catalog :
105267
citations: 3
Reference
Phetruen T, van Dam B, Chanarat S. Andrographolide Induces ROS-Mediated Cytotoxicity, Lipid Peroxidation, and Compromised Cell Integrity in Saccharomyces cerevisiae. Antioxidants (Basel). 2023;12: pubmed publisher
Heckle L, Kozminski K. Osh-dependent and -independent Regulation of PI4P Levels During Polarized Growth of Saccharomyces cerevisiae. Mol Biol Cell. 2023;34:ar104 pubmed publisher
Day K, Casler J, Glick B. Budding Yeast Has a Minimal Endomembrane System. Dev Cell. 2018;44:56-72.e4 pubmed publisher
product information
Catalog Number :
105267
Product Name :
YIplac211-SEC7-mCherry2Bx6
article :
doi10.1016/j.devcel.2017.12.014
id28192448
pubmed_id29316441
bacterial resistance :
Ampicillin
cloning :
backboneYIplac211
backbone_mutation
backbone_origin
backbone_size3797
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Addgene's quality control identified a frameshift at Q266 of URA3. The depositing lab states that this mutation should not affect selection.
growth strain :
Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SphI
cloning_site_5EcoRI
promoter
sequencing_primer_3M13 REVERSE
sequencing_primer_5M13 FORWARD
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDR170C
geneSEC7
id851748
genbank_ids
mutation
nameSec7 portion
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagmCherry2Bx6
resistance markers :
167
tags :
Unknown
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA