pTRE-T2- mIR-IRES-Hygromycin | Addgene 105169 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTRE-T2- mIR-IRES-Hygromycin

catalog :

105169

product information

Catalog Number :

105169

Product Name :

pTRE-T2- mIR-IRES-Hygromycin

article :

doi
id	28192424
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pTRE-T2-mIR-Hygromycin

backbone_mutation

backbone_origin

Clontech = pTRE-T2

backbone_size

5041

promoter

Tet/CMV

sequencing_primer_3

TAT TAC CGC CTT TGA GTG AGC TGA

sequencing_primer_5

AGG CGT ATC ACG AGG CCC TTT CGT

vector_types

Mammalian Expression

growth notes :

A synthetic DNA containing the IRES element was cloned into EcoRI/Xba I site of pTRE-T2-miR-HYGRO vector after removal of the mIR cassette. First digest parental pTRE-T2-miR-HYGRO vector with EcoRI and Xba I to remove the miR cassette. Then the IRES cassette was ligated back to the EcoRI site and Xba I site. The IRES cassette contains the unique EcoRI site followed by unique NotI and Mlu I sites that is followed by the start of the IRES sequence. This cloning site will allow insertion of the 1st cDNA. On the 3 end of IRES there are unique Spe I and Xba I cloning sites for insertion of the second cDNA into the cassette. Please note- to use the BsaI and XbaI sites for cloning, you must use unmethylated DNA. You will need to isolate the DNA from a methylation deficient bacterial strain.

growth strain :

Mammalian expression vector for expressing tandem cDNAs

growth temp :

Methylation deficient cells from NEB to alleviate methylation sensitive restriction sites like Xba I. However not recommended for long-term propagation and storage.

origin :

resistance markers :

3629

tags :

High Copy

terms :

Hygromycin