This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRluc-LC3G120A
catalog :
105003
citations: 1
Reference
Farkas T, Jaattela M. Renilla Luciferase-LC3 Based Reporter Assay for Measuring Autophagic Flux. Methods Enzymol. 2017;588:1-13 pubmed publisher
product information
Catalog Number :
105003
Product Name :
pRluc-LC3G120A
article :
doi10.1016/bs.mie.2016.09.072
id28192380
pubmed_id28237095
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutationRluc-LC3G120A ORF inserted instead of the EGFP ORF.
backbone_originClontech
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth strain :
Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.
origin :
37
pi :
alt_names
MAP1LC3B
cloning
entrez_gene
aliasesLC3B, Map1lc3, Mpl3, zbs559
geneMap1lc3b
id64862
genbank_ids
mutationGlycine 120 mutated to alanine, prevents lipidation of the protein and thereby its binding to autophagosomal membranes
nameLC3B
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagRluc with C124A substitution
plasmid copy :
The gene was originally cloned in Tamotsu Yoshimori Laboratory, reference Kabeya et al. 2000 and addgene ID 21073.
resistance markers :
3714
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA