This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
catalog :
105000
citations: 1
Reference
Voigt F, Zhang H, Cui X, Triebold D, Liu A, Eglinger J, et al. Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Cell Rep. 2017;21:3740-3753 pubmed publisher
product information
Catalog Number :
105000
Product Name :
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
article :
doi10.1016/j.celrep.2017.12.008
id28192369
pubmed_id29281824
bacterial resistance :
Ampicillin
cloning :
backbonepSF4
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth notes :
Note plasmid contains 24xGCN4 repeats and not 20xGCN4 repeats as indicated in the name.
growth strain :
Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterTetCMV
sequencing_primer_3
sequencing_primer_5AATACGACTCACTATAGGC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameRenilla luciferase
shRNA_sequence
size930
species
32644
Other
Renilla reniformis
tags
locationN terminal on insert
tag24xGCN4 repeats (SunTag)
locationC terminal on insert
tagPreScission protease site-Rh1-RILPL2
location
tag24xPP7v3 stem loops in 3'UTR
location
tagxrRNA1-xrRNA2 in 3'UTR
location
tag24xMS2v5 stem loops in 3'UTR
resistance markers :
2309
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA