This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HLTV-hTRIM21
catalog :
104973
citations: 5
Reference
Mevissen T, Prasad A, Walter J. TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away. Cell Rep. 2023;42:112125 pubmed publisher
Kunishige R, Murata M, Kano F. Targeted protein degradation by Trim-Away using cell resealing coupled with microscopic image-based quantitative analysis. Front Cell Dev Biol. 2022;10:1027043 pubmed publisher
Chen G, Kong Y, Li Y, Huang A, Wang C, Zhou S, et al. A Promising Intracellular Protein-Degradation Strategy: TRIMbody-Away Technique Based on Nanobody Fragment. Biomolecules. 2021;11: pubmed publisher
Clift D, So C, McEwan W, James L, Schuh M. Acute and rapid degradation of endogenous proteins by Trim-Away. Nat Protoc. 2018;13:2149-2175 pubmed publisher
Clift D, McEwan W, Labzin L, Konieczny V, Mogessie B, James L, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017;171:1692-1706.e18 pubmed publisher
product information
Catalog Number :
104973
Product Name :
HLTV-hTRIM21
article :
doi10.1016/j.cell.2017.10.033
id28192365
pubmed_id29153837
bacterial resistance :
Ampicillin
cloning :
backboneHLTV
backbone_mutation
backbone_originMark Allen (MRC LMB)
backbone_size3113
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Purification of His-Lipoyl-hTRIM21 protein from bacteria
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI/BglII
promoterT7
sequencing_primer_3
sequencing_primer_5TAA TAC GAC TCA CTA TAG GG
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesRNF81, RO52, Ro/SSA, SSA, SSA1
geneTRIM21
id6737
genbank_ids
mutation
nameTripartite Motif containing 21
shRNA_sequence
size1428
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis-Lipoyl
resistance markers :
3719
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA