This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSMPP-mCherry-hTRIM21
catalog :
104972
citations: 3
Reference
Gerri C, McCarthy A, Alanis Lobato G, Demtschenko A, Bruneau A, Loubersac S, et al. Initiation of a conserved trophectoderm program in human, cow and mouse embryos. Nature. 2020;587:443-447 pubmed publisher
Clift D, So C, McEwan W, James L, Schuh M. Acute and rapid degradation of endogenous proteins by Trim-Away. Nat Protoc. 2018;13:2149-2175 pubmed publisher
Clift D, McEwan W, Labzin L, Konieczny V, Mogessie B, James L, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017;171:1692-1706.e18 pubmed publisher
product information
Catalog Number :
104972
Product Name :
pSMPP-mCherry-hTRIM21
article :
doi10.1016/j.cell.2017.10.033
id28192365
pubmed_id29153837
bacterial resistance :
Ampicillin
cloning :
backbonepSMPP
backbone_mutation
backbone_originWilliam A. McEwan (MRC LMB)
backbone_size10016
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5none
promoterSFFV
sequencing_primer_3ATAGCGTAAAAGGAGCAACATAG
sequencing_primer_5TGCTTCTCGCTTCTGTTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRNF81, RO52, Ro/SSA, SSA, SSA1
geneTRIM21
id6737
genbank_ids
mutation
nameTripartite Motif containing 21
shRNA_sequence
size1428
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmCherry
resistance markers :
3719
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA