LIC1B_Caspase-LOVN7C-7 C450A | Addgene 104628 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LIC1B_Caspase-LOVN7C-7 C450A

catalog :

104628

citations: 1

Reference
Smart A, Pache R, Thomsen N, Kortemme T, Davis G, Wells J. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci U S A. 2017;114:E8174-E8183 pubmed publisher

product information

Catalog Number :

104628

Product Name :

LIC1B_Caspase-LOVN7C-7 C450A

article :

doi	10.1073/pnas.1705064114
id	28192294
pubmed_id	28893998

bacterial resistance :

Kanamycin

cloning :

backbone

pET His6 TEV LIC cloning vector

backbone_mutation

backbone_origin

Scott Gradia (Addgene plasmid # 29653)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Please note that during Addgene's quality control process 2 mismatches were found in lacI which result in mutations S219F and Q311P. LOV2 domain was inserted into the intersubunit linker of the caspase, with linker variation N7C-7 and mutation C450A, which is a dark-locked and light-insensitive mutation

growth strain :

bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	T7
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CPP32, CPP32B, SCA-1
gene	CASP3
id	836

genbank_ids

mutation

C450A (dark-locked and light-insensitive mutation)

name

Caspase-3

shRNA_sequence

size

species

9606

Homo sapiens