This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJC-mhc2.4kb-TALE3-VP64-P10
catalog :
104611
citations: 1
Reference
Toegel M, Azzam G, Lee E, Knapp D, Tan Y, Fa M, et al. A multiplexable TALE-based binary expression system for in vivo cellular interaction studies. Nat Commun. 2017;8:1663 pubmed publisher
product information
Catalog Number :
104611
Product Name :
pJC-mhc2.4kb-TALE3-VP64-P10
article :
doi10.1038/s41467-017-01592-3
id28192040
pubmed_id29162808
bacterial resistance :
Ampicillin
cloning :
backbonepJC-20xUAS-TALE3-VP64-P10
backbone_mutation20xUAS replaced with 2.4kb mhc enhancer element
backbone_originTudor A. Fulga
backbone_size11291
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
TALEN
growth strain :
Expresses TALE3 under the control of a 2.4kb mhc enhancer element
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5HindIII
promoterhsp70
sequencing_primer_3AGGGTTAGTCGTTTCGGTGTGC
sequencing_primer_5CGGTGATTCATTCTGCTAACCA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG17927, Bsh, CG17927, DROMHC, Dm II, DmMHC, Dmel CG17927, DroMII, Ifm(2)2, Ifm(2)RU1, MHC, MRP, Mhc36B, Mrp, MyHC, Myo, Myo6, Nup, Sht, Stp, chr2L:16765388. 16765564, chr2L:16781880. 16782040, ifm(2)2, ifm(2)RU1, ifm(2)RU2, l(2)36Ae, l(2)M66, l(2)k10423, mMHC, mhc, sMHC
geneMhc
id35007
genbank_ids
NM_165190.4
mutation
name2.4kb mhc enhancer element
shRNA_sequence
size2394
species
7227
Drosophila melanogaster
tags
plasmid copy :
The mhc enhancer element was cloned from plasmid pFS209_CaSpeR_Mhc-GAL4 (gift from Frank Schnorrer) published in: Klein, P. et al. Ret rescues mitochondrial morphology and muscle degeneration of Drosophila Pink1 mutants. EMBO J 33, 341-355 (2014).
resistance markers :
3609
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA