This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBluescriptKS(-)-FRBx2
catalog :
104476
citations: 1
Reference
Papanikou E, Day K, Austin J, Glick B. COPI selectively drives maturation of the early Golgi. elife. 2015;4: pubmed publisher
product information
Catalog Number :
104476
Product Name :
pBluescriptKS(-)-FRBx2
article :
doi10.7554/eLife.13232
id28192243
pubmed_id26709839
bacterial resistance :
Ampicillin
cloning :
backbonepBlueScript KS (-)
backbone_mutation
backbone_originStratagene
backbone_size2958
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
phagemid
growth strain :
Plasmid carrying FRBx2 with C-terminal HA tag.
origin :
37
pi :
alt_names
FKBP-rapamycin binding domain
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoter
sequencing_primer_3M13 FORWARD
sequencing_primer_5M13 REVERSE
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
mutationincludes only the FRB domain
nameFRBx2
shRNA_sequence
size717
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA tag
plasmid copy :
The FRB gene was obtained from Ariad Pharmaceuticals (Cambridge, MA).
resistance markers :
167
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA