This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC1-hVAP-B KD/MD
catalog :
104450
citations: 2
Reference
Angara R, Sadi A, Gilk S. The novel bacterial effector protein CbEPF1 mediates ER-LD membrane contacts to regulate host lipid droplet metabolism. bioRxiv. 2023;: pubmed publisher
Alpy F, Rousseau A, Schwab Y, Legueux F, Stoll I, Wendling C, et al. STARD3 or STARD3NL and VAP form a novel molecular tether between late endosomes and the ER. J Cell Sci. 2013;126:5500-12 pubmed publisher
product information
Catalog Number :
104450
Product Name :
pEGFPC1-hVAP-B KD/MD
article :
doi10.1242/jcs.139295
id19674
pubmed_id24105263
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells
origin :
37
pi :
alt_names
VAP-B
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3GAGGTTTTACTTGCTTTAAA
sequencing_primer_5GATCACATGGTCCTGCTG
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesALS8, VAMP-B, VAP-B
geneVAPB
id9217
genbank_ids
NM_004738.3
mutationchanged K87 to D and M89 to D
namevesicle-associated membrane protein-associated protein B
shRNA_sequence
size732
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
locationN terminal on insert
tagHA
plasmid copy :
Kentaro Hanada (VAP-B cDNA) Reference: Kawano M et al, J Biol Chem. 2006 Oct 6;281(40):30279-88. PMID: 16895911
resistance markers :
3694
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA