This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPC1-hVAP-A KD/MD
catalog :
104449
citations: 2
Reference
Lu M, van Tartwijk F, Lin J, Nijenhuis W, Parutto P, Fantham M, et al. The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes. Sci Adv. 2020;6: pubmed publisher
Alpy F, Rousseau A, Schwab Y, Legueux F, Stoll I, Wendling C, et al. STARD3 or STARD3NL and VAP form a novel molecular tether between late endosomes and the ER. J Cell Sci. 2013;126:5500-12 pubmed publisher
product information
Catalog Number :
104449
Product Name :
pEGFPC1-hVAP-A KD/MD
article :
doi10.1242/jcs.139295
id19674
pubmed_id24105263
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells
origin :
37
pi :
alt_names
VAP-A
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3GAGGTTTTACTTGCTTTAAA
sequencing_primer_5GATCACATGGTCCTGCTG
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesVAMP-A, VAP-33, VAP-A, VAP33, hVAP-33
geneVAPA
id9218
genbank_ids
NM_194434.2
mutationchanged K94 to D and M96 to D
namevesicle-associated membrane protein-associated protein A
shRNA_sequence
size747
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
plasmid copy :
Kentaro Hanada (VAP-A cDNA). Reference: Kawano M et al, J Biol Chem. 2006 Oct 6;281(40):30279-88. PMID: 16895911
resistance markers :
3694
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA