GST-BHMT-I/G | Addgene 104442 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

GST-BHMT-I/G

catalog :

104442

citations: 3

Reference
Stefely J, Zhang Y, Freiberger E, Kwiecien N, Thomas H, Davis A, et al. Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. Autophagy. 2020;:1-19 pubmed publisher
Thomas H, Zhang Y, Stefely J, Veiga S, Thomas G, Kozma S, et al. Mitochondrial Complex I Activity Is Required for Maximal Autophagy. Cell Rep. 2018;24:2404-2417.e8 pubmed publisher
Mercer C, Kaliappan A, Dennis P. Macroautophagy-dependent, intralysosomal cleavage of a betaine homocysteine methyltransferase fusion protein requires stable multimerization. Autophagy. 2008;4:185-94 pubmed

product information

Catalog Number :

104442

Product Name :

GST-BHMT-I/G

article :

doi	5275 [pii]
id	28192231
pubmed_id	18059170

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5

backbone_mutation

backbone_origin

backbone_size

4754

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

GFP is from Aequorea victoria

growth strain :

Autophagy cargo assay

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	Nru1
promoter	CMV
sequencing_primer_3
sequencing_primer_5	SP6 5'-TGATGAGTTTGGACAAACCAC-3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BHMT1, HEL-S-61p
gene	BHMT
id	635

genbank_ids

mutation

N43A in GST (in a potential KFERQ-like sequence). BHMT does not contain the first 8 or last 9 amino acids.

name

BHMT

shRNA_sequence

size

3213

species

9606

Homo sapiens