p-dCas9-LSD1-K661A-Hygro | Addgene 104414 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p-dCas9-LSD1-K661A-Hygro

catalog :

104414

product information

Catalog Number :

104414

Product Name :

p-dCas9-LSD1-K661A-Hygro

article :

doi
id	28192225
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pMLM3705

backbone_mutation

backbone_origin

Keith Joung (Addgene plasmid # 47754)

backbone_size

9075

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth notes :

Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). LSD1A isoform B catalytic domain (a.a. 171-852) was from LSD1 cDNA clone IRATp970A0364D (Source Bioscience). The P405H change in this cDNA clone was reverted to the consensus during Gibson assembly. K661A mutation was introduced by PCR.

growth strain :

transient expression of dCas9-dLSD1 fusion protein

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CMV promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AOF2, BHC110, CPRF, KDM1, LSD1
gene	KDM1A
id	23028

genbank_ids

mutation

IRATp970A0364D (Source Bioscience) has a P405H change that was reverted to the consensus. K661A

name

dLSD1

shRNA_sequence

size

species

9606

Homo sapiens