This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p-dCas9-HDAC1-Hygro
catalog :
104409
product information
Catalog Number :
104409
Product Name :
p-dCas9-HDAC1-Hygro
article :
doi
id28192225
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepMLM3705
backbone_mutation
backbone_originKeith Joung (Addgene plasmid # 47754)
backbone_size9075
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). HDAC1 (a.a. 4-384) was from Addgene plasmid 13820 (HDAC1 Flag)
growth strain :
transient expression of dCas9-HDAC1 fusion protein
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGON-10, HD1, KDAC1, RPD3, RPD3L1
geneHDAC1
id3065
genbank_ids
mutation
nameHDAC1
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
3678
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA