This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJO629
catalog :
104342
citations: 2
Reference
Oh J, Chen S, Varshavsky A. A reference-based protein degradation assay without global translation inhibitors. J Biol Chem. 2017;292:21457-21465 pubmed publisher
Oh J, Hyun J, Varshavsky A. Control of Hsp90 chaperone and its clients by N-terminal acetylation and the N-end rule pathway. Proc Natl Acad Sci U S A. 2017;114:E4370-E4379 pubmed publisher
product information
Catalog Number :
104342
Product Name :
pJO629
article :
doi10.1074/jbc.M117.814236
id28192195
pubmed_id29122887
bacterial resistance :
Ampicillin
cloning :
backbonepRS313
backbone_mutation
backbone_origin
backbone_size4967
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
The 3788 bp insert covers entire PRT module.For cloning of gene of interest, just use Asc I/Bam HI sites.
growth strain :
Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
origin :
37
pi :
alt_names
PRT (promoter reference technique)
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5AscI
promoter
sequencing_primer_3M13-reverse
sequencing_primer_5M13-forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYIR018W
geneYAP5
id854835
genbank_ids
mutation
namepTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1
shRNA_sequence
size3788
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tag3xFLAG
resistance markers :
545
tags :
Low Copy
terms :
HIS3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA