This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GST-AR-1-173
catalog :
104193
citations: 1
Reference
He B, Kemppainen J, Wilson E. FXXLF and WXXLF sequences mediate the NH2-terminal interaction with the ligand binding domain of the androgen receptor. J Biol Chem. 2000;275:22986-94 pubmed
product information
Catalog Number :
104193
Product Name :
GST-AR-1-173
article :
doi10.1074/jbc.M002807200
id28192170
pubmed_id10816582
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-5x-1
backbone_mutation
backbone_originPharmacia
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The GST-AR insert contains an additional 4 glutamine residues at amino acid 74 compared to AAA51886.1 and the depositor has confirmed that this does not impact plasmid function.
growth strain :
Expresses human AR aa 1-173 in pGEX-5x-1 bacterial expression vector with GST
inserts :
XL-1 blue
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamH1
promoter
sequencing_primer_3don't have
sequencing_primer_5don't have
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
geneAR
id367
genbank_ids
AAA51886.1
mutation
namehuman androgen receptor aa 1-173
shRNA_sequence
size510
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
3305
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA