This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cherry-HA-KCC2
catalog :
104077
citations: 1
Reference
Kahle K, Merner N, Friedel P, Silayeva L, Liang B, Khanna A, et al. Genetically encoded impairment of neuronal KCC2 cotransporter function in human idiopathic generalized epilepsy. EMBO Rep. 2014;15:766-74 pubmed publisher
product information
Catalog Number :
104077
Product Name :
Cherry-HA-KCC2
article :
doi10.15252/embr.201438840
id28192134
pubmed_id24928908
bacterial resistance :
Kanamycin
cloning :
backbonepmCherry-C1
backbone_mutationCMV promoter replaced by UbC promoter
backbone_originClontech
backbone_size4731
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
origin :
37
pi :
alt_names
Slc12a5
cloning
clone_methodRestriction Enzyme
cloning_site_3Eco R1
cloning_site_5unknown
promoterUbC
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKcc2
geneSlc12a5
id171373
genbank_ids
mutationEcoR1 site in rat KCC2 was removed by silent mutation, A3267G in rat coding sequence
nameKCC2 (b isoform)
shRNA_sequence
size3350
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagmCherry-HA tag
resistance markers :
3662
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA