KCC2-pHext | Addgene 104076 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

KCC2-pHext

catalog :

104076

citations: 1

Reference
Friedel P, Ludwig A, Pellegrino C, Agez M, Jawhari A, Rivera C, et al. A Novel View on the Role of Intracellular Tails in Surface Delivery of the Potassium-Chloride Cotransporter KCC2. Eneuro. 2017;4: pubmed publisher

product information

Catalog Number :

104076

Product Name :

KCC2-pHext

article :

doi	eN-NWR-0055-17 [pii]
id	28192133
pubmed_id	28785725

bacterial resistance :

Kanamycin

cloning :

backbone

EGFP-C1

backbone_mutation

CMV promoter replaced by UbC promoter

backbone_origin

Clontech

backbone_size

4807

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

In order to directly visualize the insertion of KCC2 IGE variants into the membranes of single neurons, we created a novel construct encoding KCC2 with a pHluorine tag introduced into the second extracellular loop of the transporter , KCC2-pHex. See attached Scheme of KCC2-ext.pdf

growth strain :

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

origin :

pi :

alt_names

Slc12a5

cloning

clone_method	Restriction Enzyme
cloning_site_3	Eco R1
cloning_site_5	Hind III
promoter	UbC
sequencing_primer_3
sequencing_primer_5	UbC TGAAGCTCCGGTTTTGAACT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Kcc2
gene	Slc12a5
id	171373

genbank_ids

mutation

EcoR1 site in rat KCC2 was removed by silent mutation, A3267G in rat coding sequence. Two sets of silent mutations render construct resistant to shRNA K10 (~3482) and K11(~4793).

name

KCC2 (b isoform)

shRNA_sequence

size

3350

species

10116

Rattus norvegicus