pVZ_PcoaT_SQE1_THAS1 | Addgene 103859 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pVZ_PcoaT_SQE1_THAS1

catalog :

103859

citations: 1

Reference
Loeschcke A, Dienst D, Wewer V, Hage Hülsmann J, Dietsch M, Kranz Finger S, et al. The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis. PLoS ONE. 2017;12:e0189816 pubmed publisher

product information

Catalog Number :

103859

Product Name :

pVZ_PcoaT_SQE1_THAS1

article :

doi	10.1371/journal.pone.0189816
id	28192117
pubmed_id	29281679

bacterial resistance :

Kanamycin and Spectinomycin

cloning :

backbone

pVZ-spec

backbone_mutation

pVZ-spec is a modified version of pVZ321 (Sergey V. Shestakov) made by Anne Karradt in the lab of Wolfgang Lockau.

backbone_origin

Sergey V. Shestakov and Anne Karradt and Wolfgang Lockau

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

Synthetic Biology

growth notes :

second gene: squalene epoxidase (SQE1) third gene: coaR repressor protein from Synechoccystis sp. PCC6803 growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C

growth strain :

Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803

origin :

pi :

alt_names

THAS1

At5g48010

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	SalI
promoter	PcoaT (from Synechocystis sp. PCC 6803)
sequencing_primer_3
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT5G48010, Arabidopsis thaliana thalianol synthase 1, AtTHAS1, MDN11.9, MDN11_9, THALIANOL SYNTHASE, THAS, thalianol synthase 1
gene	THAS1
id	834852

genbank_ids

mutation

inserted genes are codon optimized. 2nd amino acid of SQE1 changed from Glu to Ala

name

thalianol synthase 1

shRNA_sequence

size

species

3702

Arabidopsis thaliana