This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pVZ_PcoaT_SQE1_CAS1
catalog :
103856
citations: 1
Reference
Loeschcke A, Dienst D, Wewer V, Hage Hülsmann J, Dietsch M, Kranz Finger S, et al. The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis. PLoS ONE. 2017;12:e0189816 pubmed publisher
product information
Catalog Number :
103856
Product Name :
pVZ_PcoaT_SQE1_CAS1
article :
doi10.1371/journal.pone.0189816
id28192117
pubmed_id29281679
bacterial resistance :
Kanamycin and Spectinomycin
cloning :
backbonepVZ-spec
backbone_mutationpVZ-spec is a modified version of pVZ321 (Sergey V. Shestakov) made by Anne Karradt in the lab of Wolfgang Lockau.
backbone_originSergey V. Shestakov and Anne Karradt and Wolfgang Lockau
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Synthetic Biology
growth notes :
second gene: squalene epoxidase (SQE1) third gene: coaR repressor protein from Synechoccystis sp. PCC6803 growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C
growth strain :
Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803
origin :
37
pi :
alt_names
CAS1
At2g07050
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterPcoaT (from Synechocystis sp. PCC 6803)
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G07050, CYCLOARTENOL SYNTHASE 1, T4E14.16, T4E14_16, cycloartenol synthase 1
geneCAS1
id815275
genbank_ids
mutationinserted genes are codon optimized. 2nd amino acid of SQE1 changed from Glu to Ala
namecycloartenol synthase 1
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
resistance markers :
3657
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA