pmCherry-MS2coatProtein | Addgene 103832 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pmCherry-MS2coatProtein

catalog :

103832

citations: 1

Reference
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. Real-time observation of light-controlled transcription in living cells. J Cell Sci. 2017;130:4213-4224 pubmed publisher

product information

Catalog Number :

103832

Product Name :

pmCherry-MS2coatProtein

article :

doi	10.1242/jcs.205534
id	28192115
pubmed_id	29122982

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

3941

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

EGFP was cut out from the backbone (AgeI/BamHI cuts); relevance of mutations unknown, but insert sequence is almost identical (except A88G, I30V) to another published MCP sequence (GenBank: JQ624676.1, Auslaender et al. 2012, Nature 487(7405): 123-127)

growth strain :

Live cell reader for MS2 loop RNA production

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	AgeI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MS2g2
gene	cp
id	1260899

genbank_ids

mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G), A246C (silent, A82A), T291C (silent, A97A)

name

mCherry-MS2 coat protein

shRNA_sequence

size

1102

species

32644

Other