This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ptagRFP-MS2coatProtein
catalog :
103831
citations: 2
Reference
Zuo L, Zhang G, Massett M, Cheng J, Guo Z, Wang L, et al. Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription. Nat Commun. 2021;12:1491 pubmed publisher
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. Real-time observation of light-controlled transcription in living cells. J Cell Sci. 2017;130:4213-4224 pubmed publisher
product information
Catalog Number :
103831
Product Name :
ptagRFP-MS2coatProtein
article :
doi10.1242/jcs.205534
id28192115
pubmed_id29122982
bacterial resistance :
Kanamycin
cloning :
backbonepTagRFP_C
backbone_mutation
backbone_originClontech
backbone_size4678
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
relevance of mutations unknown, but insert sequence is almost identical (except A88G, I30V) to another published MCP sequence (GenBank: JQ624676.1, Auslaender et al. 2012, Nature 487(7405): 123-127)
growth strain :
Live cell reader for MS2 loop RNA production
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMS2g2
genecp
id1260899
genbank_ids
mutationMCP: nt 202-240 are missing (13 aa), C242G (A81G), A246C (silent, A82A), T291C (silent, A97A)
nameMS2 coat protein (MCP)
shRNA_sequence
size359
species
32644
Other
tags
locationN terminal on backbone
tagtagRFP
resistance markers :
3654
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA