This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPHR-EYFP-hGCN5-NLS
catalog :
103827
citations: 1
Reference
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. Real-time observation of light-controlled transcription in living cells. J Cell Sci. 2017;130:4213-4224 pubmed publisher
product information
Catalog Number :
103827
Product Name :
pPHR-EYFP-hGCN5-NLS
article :
doi10.1242/jcs.205534
id28192115
pubmed_id29122982
bacterial resistance :
Kanamycin
cloning :
backbonepmCherry-N1
backbone_mutation
backbone_originClontech
backbone_size3935
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
mCherry was cut out from the backbone (XhoI/XbaI cuts); relevance of mutations unknown; amplified by PCR from Addgene #14106, NLS inserted by PCR
growth strain :
Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
origin :
37
pi :
alt_names
PHR domain of CRY2
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2
geneCRY2
id839529
aliasesGCN5, GCN5L2, PCAF-b, hGCN5
geneKAT2A
id2648
genbank_ids
mutationhGCN5: A566G (E189G), G585T (K195N), G726A (silent, K242K)
namePHR-EYFP-hGCN5-NLS
shRNA_sequence
size4776
species
9606
Homo sapiens
3702
Arabidopsis thaliana
tags
resistance markers :
3654
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA