pCIBN-LacI-tagRFP-T | Addgene 103810 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCIBN-LacI-tagRFP-T

catalog :

103810

citations: 1

Reference
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. Real-time observation of light-controlled transcription in living cells. J Cell Sci. 2017;130:4213-4224 pubmed publisher

product information

Catalog Number :

103810

Product Name :

pCIBN-LacI-tagRFP-T

article :

doi	10.1242/jcs.205534
id	28192115
pubmed_id	29122982

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

3932

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

EGFP was cut out from the backbone (NheI/BamHI cuts); relevance of mutations unknown

growth strain :

'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

origin :

pi :

alt_names

N-terminus of CIB1

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NheI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1
gene	CIB1
id	829604

aliases	O3K_19750
gene	lacI
id	13702623

genbank_ids

mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C370A (L124I), C404T (T135I), A417T (silent, A139A), G424A (A142T), T465A (silent, T155T)

name

CIBN-LacI-tagRFP-T

shRNA_sequence

size

2337

species

3702

Arabidopsis thaliana

32644

Other

E. coli