This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCIBN-EYFP-TetR
catalog :
103798
citations: 2
Reference
Trojanowski J, Rademacher A, Erdel F, Rippe K. Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells. Methods Mol Biol. 2019;2038:251-270 pubmed publisher
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. Real-time observation of light-controlled transcription in living cells. J Cell Sci. 2017;130:4213-4224 pubmed publisher
product information
Catalog Number :
103798
Product Name :
pCIBN-EYFP-TetR
article :
doi10.1242/jcs.205534
id28192115
pubmed_id29122982
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size3932
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
EGFP was cut out from the backbone (NheI/BamHI cuts); relevance of mutations unknown
growth strain :
BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
origin :
37
pi :
alt_names
N-terminus of CIB1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1
geneCIB1
id829604
aliasesTC1_0054
genetetR
id13877205
genbank_ids
mutationTetR: A4G (M2V)
nameCIBN-EYFP-TetR
shRNA_sequence
size1937
species
3702
Arabidopsis thaliana
32644
Other
E. coli
tags
resistance markers :
3654
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA