This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
dCas-hHDAC3-R265P
catalog :
103786
citations: 1
Reference
Kwon D, Zhao Y, Lamonica J, Zhou Z. Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC. Nat Commun. 2017;8:15315 pubmed publisher
product information
Catalog Number :
103786
Product Name :
dCas-hHDAC3-R265P
article :
doi10.1038/ncomms15315
id28189744
pubmed_id28497787
bacterial resistance :
Ampicillin
cloning :
backboneplenti
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
The stop codon present after the HDAC3 open reading frame may interfere with expression of the blasticidin resistance cassette. Blasticidin selection has not been verified with this plasmid
growth strain :
Expresses dCas9 fused to human HDAC3 with R265P mutation
origin :
37
pi :
alt_names
histone deacetylase 3
cloning
clone_methodRestriction Enzyme
cloning_site_3BsrGI
cloning_site_5BamHI
promoterEF1A
sequencing_primer_3
sequencing_primer_5GTTTGGATCTTGGTTCATTCTCAAGCCTCAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHD3, KDAC3, RPD3, RPD3-2
geneHDAC3
id8841
genbank_ids
NM_003883
mutationChanged arginine 265 to proline
namedCas9-HDAC3-R265P
shRNA_sequence
size
species
9606
Homo sapiens
100
Synthetic
32644
Other
S. pyogenes
tags
plasmid copy :
human HDAC3 cDNA from Addgene plasmid HDAC3-Flag (#13819) plenti vector containing dCas9_2A_Blast from Addgene plasmid (#61425)
resistance markers :
3595
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA