LSB-hsa-miR-16-1-3p | Addgene 103270 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LSB-hsa-miR-16-1-3p

catalog :

103270

citations: 1

Reference
Gam J, Babb J, Weiss R. A mixed antagonistic/synergistic miRNA repression model enables accurate predictions of multi-input miRNA sensor activity. Nat Commun. 2018;9:2430 pubmed publisher

product information

Catalog Number :

103270

Product Name :

LSB-hsa-miR-16-1-3p

article :

doi	10.1038/s41467-018-04575-0
id	28192107
pubmed_id	29934631

bacterial resistance :

Ampicillin and Kanamycin

cloning :

backbone

Low Sensor Backbone (LSB)

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Synthetic Biology

growth notes :

Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.

growth strain :

Used to sense miRNA activity using fluorescence based tools. hsa-miR-16-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	EF-1a
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIRN16-1, miRNA16-1, mir-16-1
gene	MIR16-1
id	406950

genbank_ids

mutation

name

hsa-miR-16-1-3p target

shRNA_sequence

size

species

9606

Homo sapiens