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product summary
company name :
Addgene
product type :
cDNA
product name :
pKSJ376
catalog :
103065
citations: 1
product information
Catalog Number :
103065
Product Name :
pKSJ376
article :
| doi | 10.1128/JB.00412-16 |
| id | 28192098 |
| pubmed_id | 27795317 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pET52-b | |
| backbone_mutation | ||
| backbone_origin | Novagen EMD/Millipore | |
| backbone_size | 5627 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter.
growth strain :
Expresses colicin E1 with R domain deletion and ImmE1
growth temp :
use BL21(DE3) for protein expression. Expression was done using Millipore Overnight Express Instant TB Medium, which self-induces
inserts :
BL21(DE3)
origin :
37
pi :
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
resistance markers :
| 865 |
| 3670 |
tags :
High Copy
terms :
| Other |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments