pKSJ375 | Addgene 103064 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pKSJ375

catalog :

103064

citations: 1

Reference
Jakes K. The Colicin E1 TolC Box: Identification of a Domain Required for Colicin E1 Cytotoxicity and TolC Binding. J Bacteriol. 2017;199: pubmed publisher

product information

Catalog Number :

103064

Product Name :

pKSJ375

article :

doi	10.1128/JB.00412-16
id	28192098
pubmed_id	27795317

bacterial resistance :

Ampicillin

cloning :

backbone

pET52-b

backbone_mutation

backbone_origin

Novagen/EMD Millipore

backbone_size

5227

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

This protein was never expressed independently. It was cloned in order to subsequently clone a colicin gene, which would be lethal in the absence of the immunity protein. The cloned fragment was from base pair 500 to base pair 48 of ColE1, which encompasses the E1 immunity protein promoter and the gene.

growth strain :

Expresses ImmE1, immunity to colicin E1

growth temp :

use BL21(DE3) for protein expression

inserts :

BL21(DE3)

origin :

pi :

alt_names

cei

cloning

clone_method	Restriction Enzyme
cloning_site_3	AvrII
cloning_site_5	AvrII
promoter	E1 immunity protein promoter from ColE1
sequencing_primer_3	T7-term
sequencing_primer_5	T7-term
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	pColE1_26
gene	pColE1_26
id	2693957

aliases	pColE1H22_p2
gene	imm
id	8656959

genbank_ids

J01566.1

mutation

name

colicin E1 immunity protein

shRNA_sequence

size

440

species

32644

Other

E. coli