This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUDE710
catalog :
103020
citations: 2
Reference
Randazzo P, Bennis N, Daran J, Daran Lapujade P. gEL DNA: A Cloning- and Polymerase Chain Reaction-Free Method for CRISPR-Based Multiplexed Genome Editing. CRISPR J. 2021;: pubmed publisher
Swiat M, Dashko S, den Ridder M, Wijsman M, van der Oost J, Daran J, et al. FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae. Nucleic Acids Res. 2017;45:12585-12598 pubmed publisher
product information
Catalog Number :
103020
Product Name :
pUDE710
article :
doi10.1093/nar/gkx1007
id28192080
pubmed_id29106617
bacterial resistance :
Ampicillin
cloning :
backbonepUD628
backbone_mutation
backbone_origin wiat M. Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR
backbone_size4995
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
CRISPR
growth strain :
expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)
origin :
37
pi :
GATGCATCTTGGCTAGCAATGAACAGAGAATTTCTACTGTTGTAGATGsizespecies
4932
Saccharomyces cerevisiae
tags
Cloningcloning_site_3
cloning_site
_5promotertd>SNR52sequencin
g_primer_3se
quencing_primer_5CCGGTTGTGGTAT
ATTTGGTGsite_3_destro
yedsite_5_de
stroyed
entrez_gene>>YOR128C
aliases
gene
ADE2
id854295
ble>aliasesYCL030C>geneHIS4r>id850327table><
td>genbank_ids
N
M_001183547
NM_0011786
75
m
utationname<
/td>crADE2-3.crHIS4-4.Sr>shRNA_sequenceAATTTCTACT
GTTGTAGATCCGGTTGTGGTATATTTGGTGTGGAAATTT
CTACTGTTGTA
alt_names
cloning
clone_methodGibson
resistance markers :
3594
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA