PresentER-SIINFEKL (mCherry) | Addgene 102945 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PresentER-SIINFEKL (mCherry)

catalog :

102945

citations: 3

Reference
Su X, Xu Y, Fox G, Xiang J, Kwakwa K, Davis J, et al. Breast cancer-derived GM-CSF regulates arginase 1 in myeloid cells to promote an immunosuppressive microenvironment. J Clin Invest. 2021;131: pubmed publisher
Gejman R, Jones H, Klatt M, Chang A, Oh C, Chandran S, et al. Identification of the Targets of T-cell Receptor Therapeutic Agents and Cells by Use of a High-Throughput Genetic Platform. Cancer Immunol Res. 2020;8:672-684 pubmed publisher
Gejman R, Chang A, Jones H, DiKun K, Hakimi A, Schietinger A, et al. Rejection of immunogenic tumor clones is limited by clonal fraction. elife. 2018;7: pubmed publisher

product information

Catalog Number :

102945

Product Name :

PresentER-SIINFEKL (mCherry)

article :

doi	10.7554/eLife.41090
id	28202711
pubmed_id	30499773

bacterial resistance :

Ampicillin

cloning :

backbone

PresentER

backbone_mutation

backbone_origin

backbone_size

8200

promoter

LTR

sequencing_primer_3

sequencing_primer_5

GTTCGACCCCGCCTCGATCC

vector_types

Mammalian Expression

Retroviral

growth notes :

Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint.

growth strain :

PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

origin :

pi :

alt_names

Chicken Ovalbumin H2-Kd epitope 257-264

OVA257-264

OVAL

cloning

clone_method	Restriction Enzyme
cloning_site_3	SfiI
cloning_site_5	SfiI
promoter	LTR
sequencing_primer_3
sequencing_primer_5	GTTCGACCCCGCCTCGATCC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OVA, SERPINB14
gene	OVAL
id	396058

genbank_ids

mutation

OVA257-264

name

SIINFEKL

shRNA_sequence

size

species

9031

Gallus gallus

100

Synthetic

32644

Other