This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PresentER-SIINFEKL (GFP)
catalog :
102944
citations: 7
Reference
Wang Z, Ahmed S, Labib M, Wang H, Wu L, Bavaghar Zaeimi F, et al. Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting. Nat Biomed Eng. 2023;7:1188-1203 pubmed publisher
Chen H, Ho Y, Mezzadra R, Adrover J, Smolkin R, Zhu C, et al. Senescence rewires microenvironment sensing to facilitate anti-tumor immunity. Cancer Discov. 2022;: pubmed publisher
Lei X, Lin H, Wang J, Ou Z, Ruan Y, Sadagopan A, et al. Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression. Nat Commun. 2022;13:3882 pubmed publisher
Wang Z, Ahmed S, Labib M, Wang H, Hu X, Wei J, et al. Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting. Nat Biomed Eng. 2022;6:108-117 pubmed publisher
Klatt M, Aretz Z, Curcio M, Gejman R, Jones H, Scheinberg D. An input-controlled model system for identification of MHC bound peptides enabling laboratory comparisons of immunopeptidome experiments. J Proteomics. 2020;228:103921 pubmed publisher
Gejman R, Jones H, Klatt M, Chang A, Oh C, Chandran S, et al. Identification of the Targets of T-cell Receptor Therapeutic Agents and Cells by Use of a High-Throughput Genetic Platform. Cancer Immunol Res. 2020;8:672-684 pubmed publisher
Gejman R, Chang A, Jones H, DiKun K, Hakimi A, Schietinger A, et al. Rejection of immunogenic tumor clones is limited by clonal fraction. elife. 2018;7: pubmed publisher
product information
Catalog Number :
102944
Product Name :
PresentER-SIINFEKL (GFP)
article :
doi10.7554/eLife.41090
id28202711
pubmed_id30499773
bacterial resistance :
Ampicillin
cloning :
backbonePresentER
backbone_mutation
backbone_origin
backbone_size8300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint. Please note that the Addgene sequencing result identifies a V163A mutation within EGFP that does not affect fluorescence.
growth strain :
PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)
origin :
37
pi :
alt_names
Chicken Ovalbumin H2-Kd epitope 257-264
OVA257-264
OVAL
cloning
clone_methodRestriction Enzyme
cloning_site_3SfiI
cloning_site_5SfiI
promoterLTR
sequencing_primer_3
sequencing_primer_5GTT CGA CCC CGC CTC GAT CC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOVA, SERPINB14
geneOVAL
id396058
genbank_ids
mutationOVA257-264
nameSIINFEKL
shRNA_sequence
size24
species
9031
Gallus gallus
100
Synthetic
32644
Other
tags
resistance markers :
3785
tags :
High Copy
terms :
Puromycin
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA