PB-EEA-5g-OSK2M2L1-PGK-Puro | Addgene 102909 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PB-EEA-5g-OSK2M2L1-PGK-Puro

catalog :

102909

citations: 2

Reference
Smith C, Castanon O, Said K, Volf V, Khoshakhlagh P, Hornick A, et al. Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Res. 2020;48:5183-5195 pubmed publisher
Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti E, et al. Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018;9:2643 pubmed publisher

product information

Catalog Number :

102909

Product Name :

PB-EEA-5g-OSK2M2L1-PGK-Puro

article :

doi	10.1038/s41467-018-05067-x
id	28192037
pubmed_id	29980666

bacterial resistance :

Ampicillin

cloning :

backbone

PB-GG-PGK-puro

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth strain :

PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	U6
sequencing_primer_3
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

aliases	ANOP3, MCOPS3
gene	SOX2
id	6657

aliases	EZF, GKLF
gene	KLF4
id	9314

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

aliases	CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A
gene	LIN28A
id	79727

genbank_ids

mutation

name

EEA-5g-OSK2M2L1-gRNAs-PGK-Puro

shRNA_sequence

15 gRNAs, see full sequence

size

species

9606

Homo sapiens

32644

Other