This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pG23A
catalog :
102884
citations: 1
Reference
Papagiannakis A, Niebel B, Wit E, Heinemann M. Autonomous Metabolic Oscillations Robustly Gate the Early and Late Cell Cycle. Mol Cell. 2017;65:285-295 pubmed publisher
product information
Catalog Number :
102884
Product Name :
pG23A
article :
doi10.1016/j.molcel.2016.11.018
id25648
pubmed_id27989441
bacterial resistance :
Ampicillin
cloning :
backboneHO-poly-KanMX4-HO
backbone_mutation
backbone_originhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/
backbone_size2531
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.
origin :
37
pi :
alt_names
OAF3
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNative promoter
sequencing_primer_3ggtcacagcttgtctgtaa
sequencing_primer_5ATGACGAATCCATGCAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYFR028C, OAF3
geneCDC14
id850585
genbank_ids
mutation
nameCdc14/mCherry-AID(71-114)-NatMX
shRNA_sequence
size2328
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagmCherry
locationC terminal on insert
tagAID(71-114)
plasmid copy :
The AID(71-114) sequence was retrieved from: Morawska, M., and Ulrich, H.D. (2013). An expanded tool kit for the auxin-inducible degron system in budding yeast. Yeast 30, 341 351. mCherry was retrieved from the pBS35 plasmid.
resistance markers :
3411
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA